The C2B Domain Is the Primary Ca2+ Sensor in DOC2B: A Structural and Functional Analysis

JOURNAL OF MOLECULAR BIOLOGY

卷:425

Article

Giladi, Moshe¹  Michaeli, Lirin²  Almagor, Lior¹  Bar-On, Dana^2,3  Buki, Tal¹  Ashery, Uri^2,3  Khananshvili, Daniel¹  Hirsch, Joel A.⁴ 

[1] Tel Aviv Univ, Sackler Sch Med, Dept Physiol & Pharmacol, IL-69978 Ramat Aviv, Israel

[2] Tel Aviv Univ, Dept Neurobiol, George S Wise Fac Life Sci, IL-69978 Ramat Aviv, Israel

[3] Tel Aviv Univ, Sagol Sch Neurosci, IL-69978 Ramat Aviv, Israel

[4] Tel Aviv Univ, George S Wise Fac Life Sci, Inst Biol Struct, Dept Biochem & Mol Biol, IL-69978 Ramat Aviv, Israel

关键词: C2 domain;    DOC2B;    exocytosis;    synaptic transmission;    small-angle X-ray scattering;    X-ray crystallography;   

DOI  :  10.1016/j.jmb.2013.08.017

来源: Elsevier

【 摘 要 】

DOC2B (double-C-2 domain) protein is thought to be a high-affinity Ca2+ sensor for spontaneous and asynchronous neurotransmitter release. To elucidate the molecular features underlying its physiological role, we determined the crystal structures of its isolated C2A and C2B domains and examined their Ca2+-binding properties. We further characterized the solution structure of the tandem domains (C2AB) using small-angle X-ray scattering. In parallel, we tested structure-function correlates with live cell imaging tools. We found that, despite striking structural similarity, C2B binds Ca2+ with considerably higher affinity than C2A. The C2AB solution structure is best modeled as two domains with a highly flexible orientation and no difference in the presence or absence of Ca2+. In addition, kinetic studies of C2AB demonstrate that, in the presence of unilamellar vesicles, Ca2+ binding is stabilized, as reflected by the similar to 10-fold slower rate of Ca2+ dissociation than in the absence of vesicles. In cells, isolated C2B translocates to the plasma membrane (PM) with an EC50 of 400 nM while the C2A does not translocate at submicromolar Ca2+ concentrations, supporting the biochemical observations. Nevertheless, C2AB translocates to the PM with an similar to 2-fold lower EC50 and to a greater extent than C2B. Our results, together with previous studies, reveal that the C2B is the primary Ca2+ sensing unit in DOC2B, whereas C2A enhances the interaction of C2AB with the PM. (C) 2013 Elsevier Ltd. All rights reserved.

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