Subunit Exchange in Protein Complexes

JOURNAL OF MOLECULAR BIOLOGY

卷:430

Review

Tusk, Samuel E.¹  Delalez, Nicolas J.²  Berry, Richard M.¹ 

[1] Univ Oxford, Clarendon Lab, Dept Phys, Parks Rd, Oxford OX1 3PU, England

[2] Univ Oxford, Dept Engn Sci, Parks Rd, Oxford OX1 3PJ, England

关键词: protein exchange;    bacterial flagellar motor;    stator;    type III secretion system;    FRAP;   

DOI  :  10.1016/j.jmb.2018.06.039

来源: Elsevier

【 摘 要 】

Over the past 50 years, protein complexes have been studied with techniques such as X-ray crystallography and electron microscopy, generating images which although detailed are static and homogeneous. More recently, limited application of in vivo fluorescence and other techniques has revealed that many complexes previously thought stable and compositionally uniform are dynamically variable, continually exchanging components with a freely circulating pool of spares. Here, we consider the purpose and prevalence of protein exchange, first reviewing the ongoing story of exchange in the bacterial flagella motor, before surveying reports of exchange in complexes across all domains of life, together highlighting great diversity in timescales and functions. Finally, we put this in the context of high-throughput proteomic studies which hint that exchange might be the norm, rather than an exception. (C) 2018 Elsevier Ltd. All rights reserved.

【 授权许可】

Free