期刊论文详细信息
eLife
Ligand sensing enhances bacterial flagellar motor output via stator recruitment
Soumyadeep Chakraborty1  KV Venkatesh1  Mahesh S Tirumkudulu1  Megha Agrawal1  Farha Naaz1 
[1] Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India;
关键词: bacterial flagellar motor;    stator recruitment;    ligand sensing;    E. coli;   
DOI  :  10.7554/eLife.62848
来源: eLife Sciences Publications, Ltd
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【 摘 要 】

It is well known that flagellated bacteria, such as Escherichia coli, sense chemicals in their environment by a chemoreceptor and relay the signals via a well-characterized signaling pathway to the flagellar motor. It is widely accepted that the signals change the rotation bias of the motor without influencing the motor speed. Here, we present results to the contrary and show that the bacteria is also capable of modulating motor speed on merely sensing a ligand. Step changes in concentration of non-metabolizable ligand cause temporary recruitment of stator units leading to a momentary increase in motor speeds. For metabolizable ligand, the combined effect of sensing and metabolism leads to higher motor speeds for longer durations. Experiments performed with mutant strains delineate the role of metabolism and sensing in the modulation of motor speed and show how speed changes along with changes in bias can significantly enhance response to changes in its environment.

【 授权许可】

CC BY   

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