Traveling Rocky Roads: The Consequences of Transcription-Blocking DNA Lesions on RNA Polymerase II

JOURNAL OF MOLECULAR BIOLOGY

卷:429

Article

Steurer, Barbara¹  Marteijn, Jurgen A.¹ 

[1] Erasmus MC, Dept Mol Genet, Wytemaweg 80, NL-3015 CN Rotterdam, Netherlands

关键词: RNA polymerase 2;    transcription coupled repair;    DNA damage;    nucleotide excision repair;    transcription restart;   

DOI  :  10.1016/j.jmb.2016.11.006

来源: Elsevier

【 摘 要 】

The faithful transcription of eukaryotic genes by RNA polymerase II (RNAP2) is crucial for proper cell function and tissue homeostasis. However, transcription-blocking DNA lesions of both endogenous and environmental origin continuously challenge the progression of elongating RNAP2. The stalling of RNAP2 on a transcription-blocking lesion triggers a series of highly regulated events, including RNAP2 processing to make the lesion accessible for DNA repair, R-loop-mediated DNA damage signaling, and the initiation of transcription-coupled DNA repair. The correct execution and coordination of these processes is vital for resuming transcription following the successful repair of transcription-blocking lesions. Here, we outline recent insights into the molecular consequences of RNAP2 stalling on transcription-blocking DNA lesions and how these lesions are resolved to restore mRNA synthesis. (C) 2016 The Author(s). Published by Elsevier Ltd.

【 授权许可】

Free