UVA radiation impairs phenotypic and functional maturation of human dermal dendritic cells

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:125

Article

Furio, L ; Berthier-Vergnes, O ; Ducarre, B ; Schmitt, D ; Peguet-Navarro, J

关键词: dendritic cells;    dermis;    immune suppression;    UVA;   

DOI  :  10.1111/j.0022-202X.2005.23904.x

来源: Elsevier

【 摘 要 】

There is now strong evidence that the ultraviolet A (UVA) part of the solar spectrum contributes to the development of skin cancers. Its effect on the skin immune system, however, has not been fully investigated. Here, we analyzed the effects of UVA radiation on dermal dendritic cells (DDC), which, in addition, provided further characterization of these cells. Dermal sheets were obtained from normal human skin and irradiated, or not, with UVA at 2 or 12 J per cm(2). After a 2 d incubation, the phenotype of emigrant cells was analyzed by double immunostaining and flow cytometry. Results showed that migratory DDC were best characterized by CD1c expression and that only few cells co-expressed the Langerhans cell marker Langerin. Whereas the DC extracted from the dermis displayed an immature phenotype, emigrant DDC showed increased expression of HLA-DR and acquired co-stimulation and maturation markers. We showed here that UVA significantly decreased the number of viable emigrant DDC, a process related to increased apoptosis. Furthermore, UVA irradiation impaired the phenotypic and functional maturation of migrating DDC into potent antigen-presenting cells, in a concentration-dependent manner. The results provide further evidence that UVA are immunosuppressive and suggest an additional mechanism by which solar radiation impairs immune response.

【 授权许可】

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