JOURNAL OF INVESTIGATIVE DERMATOLOGY | 卷:117 |
Cyclobutane thymidine dimers are present in human urine following sun exposure:: Quantitation using 32P-postlabeling and high-performance liquid chromatography | |
Article | |
Le Curieux, F ; Hemminki, K | |
关键词: P-32-postlabeling; creatinine; cyclobutane thymidine dimers; high-performance liquid chromatography; human urine; photoproduct; sun exposure; ultraviolet radiation; | |
DOI : 10.1046/j.1523-1747.2001.01416.x | |
来源: Elsevier | |
【 摘 要 】
Cyclobutane thymidine dimer (T=T) is the major DNA photoproduct formed in human skin after solar radiation. We have developed a P-32-postlabeling method suitable for quantitating T=T in human urine with a detection limit of about 0.5 fmol per 10 mul urine. The method was used in the present study to measure the daily T=T urinary level of two volunteers over a 15 d period, including frequent sun exposures ranging from 0 to 5 h daily. T=T was not detected before or immediately (4 h) after the initial sun exposure but was first observed in urine samples collected 18 h after the initial exposure. Thereafter, urinary T=T levels gradually increased up to a peak reached about 3 d after the maximum sun exposure. The levels decreased during the following days but were still detectable 8 d after the last sun exposure. About 70-75% decrease in excreted T=T was observed after 8 d. The T=T levels measured in urine were lower but in the same order of magnitude as the levels expected after a theoretical calculation based on previous published results and reasonable assumptions. This study shows the occurrence of cyclobutane thymidine dimers in human urine after skin exposure to solar radiation.
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10_1046_j_1523-1747_2001_01416_x.pdf | 159KB | download |