期刊论文详细信息
JOURNAL OF INVESTIGATIVE DERMATOLOGY 卷:117
Cyclobutane thymidine dimers are present in human urine following sun exposure:: Quantitation using 32P-postlabeling and high-performance liquid chromatography
Article
Le Curieux, F ; Hemminki, K
关键词: P-32-postlabeling;    creatinine;    cyclobutane thymidine dimers;    high-performance liquid chromatography;    human urine;    photoproduct;    sun exposure;    ultraviolet radiation;   
DOI  :  10.1046/j.1523-1747.2001.01416.x
来源: Elsevier
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【 摘 要 】

Cyclobutane thymidine dimer (T=T) is the major DNA photoproduct formed in human skin after solar radiation. We have developed a P-32-postlabeling method suitable for quantitating T=T in human urine with a detection limit of about 0.5 fmol per 10 mul urine. The method was used in the present study to measure the daily T=T urinary level of two volunteers over a 15 d period, including frequent sun exposures ranging from 0 to 5 h daily. T=T was not detected before or immediately (4 h) after the initial sun exposure but was first observed in urine samples collected 18 h after the initial exposure. Thereafter, urinary T=T levels gradually increased up to a peak reached about 3 d after the maximum sun exposure. The levels decreased during the following days but were still detectable 8 d after the last sun exposure. About 70-75% decrease in excreted T=T was observed after 8 d. The T=T levels measured in urine were lower but in the same order of magnitude as the levels expected after a theoretical calculation based on previous published results and reasonable assumptions. This study shows the occurrence of cyclobutane thymidine dimers in human urine after skin exposure to solar radiation.

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