Cutaneous injury induces the release of cathelicidin anti-microbial peptides active against group A Streptococcus

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:117

Article

Dorschner, RA ; Pestonjamasp, VK ; Tamakuwala, S ; Ohtake, T ; Rudisill, J ; Nizet, V ; Agerberth, B ; Gudmundsson, GH ; Gallo, RL

关键词: bacteria;    infection;    keratinocytes;    skin;    wound healing;   

DOI  :  10.1046/j.1523-1747.2001.01340.x

来源: Elsevier

【 摘 要 】

Cathelicidins are a family of peptides thought to provide an innate defensive barrier against a variety of potential microbial pathogens. The human and mouse cathelicidins (LL-37 and CRAMP, respectively) are expressed at select epithelial interfaces where they have been proposed to kill a number of gram-negative and gram-positive bacteria. To determine if these peptides play a part in the protection of skin against wound infections, the anti-microbial activity of LL-37 and CRAMP was determined against the common wound pathogen group A Streptococcus, and their expression was examined after cutaneous injury. We observed a large increase in the expression of cathelicidins in human and murine skin after sterile incision, or in mouse following infection by group A Streptococcus. The appearance of cathelicidins in skin was due to both synthesis within epidermal keratinocytes and deposition from granulocyctes that migrate to the site of injury. Synthesis and I deposition in the wound was accompanied by processing from the inactive prostorage form to the mature C-terminal peptide, Analysis of anti-microbial activity of this C-terminal peptide against group A Streptococcus revealed that both LL-37 and CRAMP potently inhibited bacterial growth. Action against group A Streptococcus occurred in conditions that typically abolish the activity of anti-microbial peptides against other organisms. Thus, cathelicidins are well suited to provide defense against infections due to group A Streptococcus, and represent an important element of cutaneous innate immunity.

【 授权许可】

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