Reproducibility and replicability of rodent phenotyping in preclinical studies

NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS

卷:87

Review

Kafkafi, Neri¹  Agassi, Joseph¹  Chesler, Elissa J.²  Crabbe, John C.^3,4  Crusio, Wim E.^5,6  Eilam, David¹  Gerlai, Robert⁷  Goiani, Ilan¹  Gomez-Marin, Alex⁸  Heller, Ruth¹  Iraqi, Fuad¹  Jaljuli, Iman¹  Karp, Natasha A.⁹  Morgan, Hugh¹⁰  Nicholson, George¹¹  Pfaff, Donald W.¹²  Richter, S. Helene¹³  Stark, Philip B.¹⁴  Stiedl, Oliver¹⁵  Stodden, Victoria¹⁶  Tarantino, Lisa M.¹⁷  Tucci, Valter¹⁸  Valdar, William¹⁷  Williams, Robert W.¹⁹  Wurbel, Hanno²⁰  Benjamini, Yoav¹ 

[1] Tel Aviv Univ, Tel Aviv, Israel

[2] Jackson Lab, 600 Main St, Bar Harbor, ME 04609 USA

[3] Oregon Hlth & Sci Univ, Portland, OR 97201 USA

[4] VA Portland Hlth Care Syst, Portland, OR USA

[5] Univ Bordeaux, INCIA, Bordeaux, France

[6] CNRS, Paris, France

[7] Univ Toronto, Toronto, ON, Canada

[8] CSIC UMH, Inst Neurociencias, Alicante, Spain

[9] AstraZeneca, Discovery Sci, IMED Biotech Unit, Cambridge, England

[10] Harwell Res Ctr, Didcot, Oxon, England

[11] Univ Oxford, Oxford, England

[12] Rockefeller Univ, 1230 York Ave, New York, NY 10021 USA

[13] Univ Munster, Munster, Germany

[14] Univ Calif Berkeley, Berkeley, CA 94720 USA

[15] Vrije Univ Amsterdam, Amsterdam, Netherlands

[16] Univ Illinois, Champaign, IL USA

[17] Univ North Carolina Chapel Hill, Chapel Hill, NC USA

[18] Ist Italiano Tecnol, Genoa, Italy

[19] Univ Tennessee, Hlth Sci Ctr, Knoxville, TN 37996 USA

[20] Univ Bern, Bern, Switzerland

关键词: Reproducibility;    Replicability;    GxE interaction;    Validity;    Data sharing;    False discoveries;    Heterogenization;   

DOI  :  10.1016/j.neubiorev.2018.01.003

来源: Elsevier

【 摘 要 】

The scientific community is increasingly concerned with the proportion of published discoveries that are not replicated in subsequent studies. The field of rodent behavioral phenotyping was one of the first to raise this concern, and to relate it to other methodological issues: the complex interaction between genotype and environment; the definitions of behavioral constructs; and the use of laboratory mice and rats as model species for investigating human health and disease mechanisms. In January 2015, researchers from various disciplines gathered at Tel Aviv University to discuss these issues. The general consensus was that the issue is prevalent and of concern, and should be addressed at the statistical, methodological and policy levels, but is not so severe as to call into question the validity and the usefulness of model organisms as a whole. Well-organized community efforts, coupled with improved data and metadata sharing, have a key role in identifying specific problems and promoting effective solutions. Replicability is closely related to validity, may affect generalizability and translation of findings, and has important ethical implications.

【 授权许可】

Free