MiR-302b regulates cell functions and acts as a potential biomarker to predict recurrence in bladder cancer

LIFE SCIENCES

卷:209

Article

Li, Zuwei^1,2,3  Zhou, Liang^1,3,4  Lin, Canbin^1,2,3  Pan, Xiang^1,3  Xie, Jiansen²  Zhao, Liwen^1,3  Quan, Jing^1,3  Xu, Jinling¹  Guan, Xin¹  Xu, Weijie¹  Li, Hang¹  Chen, Yun⁵  Lai, Yongqing^1,3 

[1] Peking Univ, Shenzhen Hosp, Dept Urol, 1120 Lianhua Rd, Shenzhen 518036, Guangdong, Peoples R China

[2] Shantou Univ, Med Coll, Dept Urol, Shantou 515041, Guangdong, Peoples R China

[3] Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen PKU HKUST Med Ctr, Shenzhen Hosp,Inst Urol, Shenzhen 518036, Guangdong, Peoples R China

[4] Guangzhou Med Univ, Dept Urol, Guangzhou 511436, Guangdong, Peoples R China

[5] Peking Univ, Shenzhen Hosp, Dept Ultrasound, 1120 Lianhua Rd, Shenzhen 518036, Guangdong, Peoples R China

关键词: microRNA;    miR-302b;    Bladder cancer;    Biomarker;   

DOI  :  10.1016/j.lfs.2018.07.057

来源: Elsevier

【 摘 要 】

Background: Bladder cancer is the most common urogenital tumor with substantial morbidity, high recurrence rate and mortality. miRNAs, a class of endogenous noncoding RNA, were found to involve in the genesis, maintenance and metastasis of cancer. Genomic profiling revealed that miR-302b is down-regulated in bladder cancer while its functions in bladder cancer remain to be ascertained. Methods: Cell functional assays including wound healing assay, CCK-8 assay, Transwell assay and flow cytometry assay were performed to clarify the functions of miR-302b expression in cell proliferation, migration, invasion and apoptosis in BC. Furthermore, RT-qPCR was performed to study the expression of miR-302b in bladder cancer tissues and the prognostic value of altered miR-302b expression with 48 formalin-fixed paraffin-embedded bladder urothelial carcinoma samples. Results: The results of RT-qPCR demonstrated that expression level of miR-302b was significantly reduced in bladder cancer tissues and cell lines. The cells after transfected with miR-302b mimic showed lower mobility, lower proliferation and increased apoptosis, while opposite results were obtained after inhibiting the expression of miR-302b. The prognosis analysis demonstrated that the patients with low expression of miR-302b experienced high risks of recurrence. Conclusions: The results of our study demonstrate that miR-302b regulates cell functions and acts as a potential biomarker to predict recurrence in bladder cancer.

【 授权许可】

Free