TECHNOLOGIES FOR QUANTITATIVE AND MULTIPLEXED BIOMARKER DETECTION

Biomarker;genetic mutation;microRNA;medical diagnosis;single molecule detection;fluorescence microscopy;nanotechnology;Biomedical Engineering

Song, YunkeWang, Tza-Huei ;

Johns Hopkins University

关键词: Biomarker;    genetic mutation;    microRNA;    medical diagnosis;    single molecule detection;    fluorescence microscopy;    nanotechnology;    Biomedical Engineering;   

Others  :  https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/40188/SONG-DISSERTATION-2014.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: JOHNS HOPKINS DSpace Repository

【 摘 要 】

Genetic and epigenetic biomarkers have enormous potential to improve healthcare management through their use for prognosis and diagnosis of diseases such as cancer. While DNA and mRNA have been studied for decades, microRNA emerges as a new class of biomarkers that promises to facilitate clinical managements of cancer as well as studies of underling mechanisms governing cancer pathogenesis. My research focuses on the development of new methods and technologies to tackle the critical barriers associated to analyses of DNA and microRNA based biomarkers. This dissertation is devoted for development of (1) genetic mutation detection method that combines excellent specificity of ligation technology and ultrahigh sensitivity of quantum dot (QD) nanosensor-based single molecule detection (SMD), (2) nanomaterial-based method for further enhancement of QD-nanosensor-based assays and (3) quantitative microRNA detection methods that are well customized to provide excellent sensitivity, specificity, multiplexing capability and handling convenience.