1,8-cineole (eucalyptol) ameliorates cerulein-induced acute pancreatitis via modulation of cytokines, oxidative stress and NF-κB activity in mice

LIFE SCIENCES

卷:92

Article

Lima, Patricia Rodrigues^1,2,3  de Melo, Tiago Sousa²  Martins Bezerra Carvalho, Karine Maria^1,4  de Oliveira, Italo Bonfim^3,4  Arruda, Bruno Rodrigues^3,4  de Castro Brito, Gerly Anne^3,5  Rao, Vietla Satyanarayana^3,4  Santos, Flavia Almeida^3,4 

[1] Univ Fed Ceara, Fac Med, Postgrad Programme Med Sci, BR-60430140 Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Dept Clin & Toxicol Anal, Fac Pharm Odontol & Nurse, BR-60430160 Fortaleza, Ceara, Brazil

[3] Univ Fed Ceara, Natl Inst Sci & Technol Biomed Brazilian Semiarid, Fac Med, BR-60430270 Fortaleza, Ceara, Brazil

[4] Univ Fed Ceara, Nat Prod Lab, Dept Physiol & Pharmacol, Fac Med, BR-60430270 Fortaleza, Ceara, Brazil

[5] Univ Fed Ceara, Dept Morphol, BR-60416030 Fortaleza, Ceara, Brazil

关键词: 1,8-cineole;    Acute pancreatitis;    Cerulein;    Inflammatory response;    Mouse model;   

DOI  :  10.1016/j.lfs.2013.05.009

来源: Elsevier

【 摘 要 】

Aims: Acute pancreatitis (AP) is an inflammatory condition wherein pro-inflammatory mediators, oxidative stress, and NF-kappa B signaling play a key role. Currently, no specific therapy exists and treatment is mainly supportive and targeted to prevent local pancreatic injury and systemic inflammatory complications. This study was aimed to examine whether 1,8-cineole, a plant monoterpene with antioxidant and anti-inflammatory properties could ameliorate cerulein-induced acute pancreatitis. Main methods: AP was induced in Swiss mice by six one hourly injections of cerulein (50 mu g/kg, i.p.). 1,8-cineole (100, 200 and 400 mg/kg, p.o.) was administered 1 h prior to first cerulein injection, keeping vehicle and thalidomide treated groups as controls: Blood samples were taken 6-h later to determine serum levels of amylase and lipase, and cytokines. The pancreas was removed for morphological examination, myeloperoxidase (MPO) and malondialdehyde (MDA) assays, reduced glutathione (GSH) levels, and for nuclear factor (NF)-kappa B immunostaining. Key findings: 1,8-cineole effectively reduced the cerulein-induced histological damage, pancreatic edema and NF-kappa B expression, levels of MPO activity and MDA, and replenished the GSH depletion. Cerulein increased serum levels of amylase and lipase, and pro-inflammatory cytokines TNF-alpha, IL-1 beta, and IL-6 were also decreased by 1,8-cineole pretreatment, similar to thalidomide, a TNF-alpha inhibitor. The anti-inflammatory IL-10 cytokine level was, however, enhanced by 1,8-cineole. Significance: These findings indicate that 1,8-cineole can attenuate cerulein-induced AP via an anti-inflammatory mechanism and by combating oxidative stress. Further studies are needed to clearly elucidate its benefits in patients on acute pancreatitis. (C) 2013 Elsevier Inc. All rights reserved.

【 授权许可】

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