Antioxidant effects of green tea and its polyphenols on bladder cells

LIFE SCIENCES

卷:83

Article

Coyle, Christian H.¹  Philips, Brian J.¹  Morrisroe, Shelby N.¹  Chancellor, Michael B.¹  Yoshimura, Naoki¹ 

[1] Univ Pittsburgh, Sch Med, Dept Urol, Pittsburgh, PA 15213 USA

关键词: green tea;    hydrogen peroxide;    cytoprotection;    polyphenols;    oxidative stress;   

DOI  :  10.1016/j.lfs.2008.04.010

来源: Elsevier

【 摘 要 】

Genitourinary tract inflammation/ailments affect the quality of life and health of a large segment of society. In recent years, studies have demonstrated strong antioxidant effects of green tea and its associated polyphenols in inflammatory states. This in vitro study examined the antioxidant capabilities (and putative mechanisms of action) of green tea extract (GTE), polyphenon-60 (PP-60, 60% pure polyphenols), (-)-epicatechin-3-gallate (ECG) and (-)-epigallocatechin-3-gallate (EGCG) in normal/malignant human bladder cells following catechin treatment +/-1 mM H2O2 (oxidative agent). Cell viability, apoptosis and reactive oxygen species (ROS) formation were evaluated. Our results showed that H2O2 exposure significantly reduced normal (UROtsa) and high-grade (TCCSUP, T24) bladder cancer (BlCa) cell viability compared with control-treated cells (p<0.001). No affect on low-grade RT4 and SW780 BlCa cell viability was observed with exposure to H2O2. Compared to H2O2-treated UROtsa, treatment with PP-60, ECG and EGCG in the presence of H2O2 significantly improved UROtsa viability (p<0.01), with strongest effects evoked by ECG. Additionally, though not as effective as in UROtsa cells, viability of both high-grade TCCSUP and T-24 BlCa cells, in comparison to H2O2-treated cells, was significantly improved (p<0.01) by treatment with PP-60, ECG, and EGCG in the presence of H2O2. Overall, our findings demonstrate that urothelium cell death via H2O2-induced oxidative stress is mediated, in part, through superoxide (O-2-.;), and potentially, direct H2O2 mechanisms, suggesting that green tea polyphenols can protect against oxidative stress/damage and bladder cell death. (C) 2008 Elsevier Inc. All rights reserved.

【 授权许可】

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