In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla

Molecules

Jesus Souza¹  Elizabeth Yamada²  Dielly Lopes²  Edmar Costa²  Paulo Sá³  Sônia Pamplona³  Milton da Silva³  Mara Arruda³  Consuelo e Silva³ 

[1] Faculdade de Engenharia de Alimentos, Instituto de Tecnologia, Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, Brazil;Laboratório de Neuropatologia Experimental (LaNEx), Hospital Universitário Barros Barreto,Rua dos Mundurucus, 4487–Guamá. CEP 66073-000. Belém, PA, Brazil;Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais,Campus Universitário do Guamá, Universidade Federal do Pará, Belém-PA 66075-970, Brazil;

关键词: Swietenia macrophylla;    cytoprotection;    antioxidant capacity;    polyphenols;    LC-MS/MS;   

DOI  :  10.3390/molecules201018777

来源: DOAJ

【 摘 要 】

Swietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards using LC-ESI-MS/MS. Polyphenols play an important role in plants as defense mechanisms against pests and pathogens and have potent antioxidant properties. In terms of health applications, interest has increased considerably in naturally occurring antioxidant sources, since they can retard the progress of many important neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. The antioxidant capacities of two aqueous extracts, M1 (decoction) and M2 (infusion), were measured using TEAC and Folin-Ciocalteau methods. Additionally, M1 was used in order to investigate its potential cytoprotective effects on an in vitro model of neurodegeneration, by using primary cerebellar cultures exposed to methyl mercury (MeHg). Under experimental sub-chronic conditions (72 h), concomitant exposure of the same cultures to MeHg and M1 extract resulted in a statistically significant increase in cell viability in all three concentrations tested (10, 50 and 100 μg/mL), strongly suggesting that due to its high content of antioxidant compounds, the M1 extract provides significant cytoprotection against the MeHg-induced in vitro neurotoxicity.

【 授权许可】

Unknown