αA-Crystallin inhibits optic nerve astrocyte activation induced by oxygen-glucose deprivation in vitro

LIFE SCIENCES

卷:278

Article

Shao, Weiyang¹  Liu, Xiao²  Gao, Lixiong¹  Tian, Chunyu¹  Shi, Qian¹ 

[1] Peoples Liberat Army Gen Hosp, Ophthalmol Dept, Med Ctr 6, Beijing 100048, Peoples R China

[2] Third Mil Med Univ, Southwest Hosp, Southwest Eye Hosp, Chongqing 400038, Peoples R China

关键词: alpha A-crystallin;    Astrocyte;    Oxygen glucose deprivation;    BMP/Smad;   

DOI  :  10.1016/j.lfs.2021.119533

来源: Elsevier

【 摘 要 】

Aims: A previous study reported that intravitreal injection of alpha A-crystallin inhibits glial scar formation after optic nerve traumatic injury. The purpose of this study was to investigate the effect of alpha A-crystallin on optic nerve astrocytes induced by oxygen glucose deprivation (OGD) in vitro. Materials and methods: Optic nerve astrocytes from newborn Long Evans rats were cultured with alpha A-crystallin (10(-4) g/l) to detect the effects of alpha A-crystallin on astrocytes. Using a scratch assay, the effect of alpha A-crystallin treatment on astrocyte migration was assessed. Astrocytes were exposed to OGD and glucose reintroduction/ reoxygenation culture for 24 h and 48 h. The expression of glial fibrillary acidic protein (GFAP) and neurocan were subsequently evaluated via immunocytochemistry and western blot. BMP2/4, BMPRIa/Ib and Smad1/5/8 mRNA expression levels were detected by RT-PCR. Key findings: The results showed that alpha A-crystallin slowed the migration of astrocytes in filling the scratch gaps. GFAP and neurocan expression in astrocytes was increased after OGD. However, after treatment with alpha A-crystallin, GFAP and neurocan expression levels clearly decreased. Furthermore, RT-PCR showed that BMP2 and BMP4 mRNA expression levels decreased significantly. Significance: These results suggest that alpha A-crystallin inhibits the activation of astrocytes after OGD injury in vitro. Inhibition of the BMP/Smad signaling pathway might be the mechanism underlying this effect.

【 授权许可】

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