Model of Oxygen and Glucose Deprivation in PC12 Cells and Detection of HSP70 Protein

5th Annual International Conference on Material Science and Environmental Engineering

材料科学;生态环境科学

He, Jinting^1 ; Yang, Le^2 ; Shao, Yankun^1

Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun

130031, China^1

China People's Hospital of Jilin Province, Changchun

130021, China^2

关键词: Culture time;    Glucose deprivation;    Ischemia model;    Neuron-like cells;    Neuronal morphology;    Oxygen glucose deprivation;    Positive correlations;    Western-blot analysis;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/301/1/012025/pdf
DOI  :  10.1088/1757-899X/301/1/012025

来源: IOP

【 摘 要 】

Objective: PC12 cell was used to set up a ischemia model by OGD and detected HSP70 protein. Methods: Use of PC12 cells induced by NGF stimulation into nerve cells, oxygen and glucose deprivation to build the nerve cells of oxygen and glucose deprivation model; using Western blot analysis of PC12 cells into neuron-like cells and oxygen-glucose deprivation model established. Results: The application of a final concentration of 50 ng / ml of NGF in DMEM complete mediumPC12 cells showed a typical neuronal morphology with the increase in cell culture time. NGF culture time showed a positive correlation, the establishment of oxygen and glucose deprivation (OGD) training environment, the OGD after nerve element appears different degrees of damage, OGD can effectively induce the expression of HSP70. Conclusion: PC12 cell transformed into cells by NGF; the cell model of OGD was established.