Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage

Cellular & Molecular Biology Letters

Raju Khatri¹  Michal Arad¹  Robert A. Brown¹  Michal Zalzman²  Rodney J. Taylor³ 

[1] Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N. Greene Street, 21201, Baltimore, MD, USA;Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N. Greene Street, 21201, Baltimore, MD, USA;The Center for Stem Cell Biology and Regenerative Medicine, University of Maryland School of Medicine, 21201, Baltimore, MD, USA;Marlene and Stewart Greenbaum Cancer Center, University of Maryland School of Medicine, 21201, Baltimore, MD, USA;Department of Otorhinolaryngology-Head and Neck Surgery, University of Maryland School of Medicine, 21201, Baltimore, MD, USA;Marlene and Stewart Greenbaum Cancer Center, University of Maryland School of Medicine, 21201, Baltimore, MD, USA;Department of Otorhinolaryngology-Head and Neck Surgery, University of Maryland School of Medicine, 21201, Baltimore, MD, USA;

关键词: Tonsil-derived multipotent stem cells (T-MSC);    Biopsies;    Neuron-like cells;    Neuroblasts;    Neuronal stem cells (NSC);    Differentiation;   

DOI  :  10.1186/s11658-021-00279-4

来源: Springer

【 摘 要 】

BackgroundNeurological disorders are considered one of the greatest burdens to global public health and a leading cause of death. Stem cell therapies hold great promise for the cure of neurological disorders, as stem cells can serve as cell replacement, while also secreting factors to enhance endogenous tissue regeneration. Adult human multipotent stem cells (MSCs) reside on blood vessels, and therefore can be found in many tissues throughout the body, including palatine tonsils. Several studies have reported the capacity of MSCs to differentiate into, among other cell types, the neuronal lineage. However, unlike the case with embryonic stem cells, it is unclear whether MSCs can develop into mature neurons.MethodsHuman tonsillar MSCs (T-MSCs) were isolated from a small, 0.6-g sample, of tonsillar biopsies with high viability and yield as we recently reported. Then, these cells were differentiated by a rapid, multi-stage procedure, into committed, post-mitotic, neuron-like cells using defined conditions.ResultsHere we describe for the first time the derivation and differentiation of tonsillar biopsy-derived MSCs (T-MSCs), by a rapid, multi-step protocol, into post-mitotic, neuron-like cells using defined conditions without genetic manipulation. We characterized our T-MSC-derived neuronal cells and demonstrate their robust differentiation in vitro.ConclusionsOur procedure leads to a rapid neuronal lineage commitment and loss of stemness markers, as early as three days following neurogenic differentiation. Our studies identify biopsy-derived T-MSCs as a potential source for generating neuron-like cells which may have potential use for in vitro modeling of neurodegenerative diseases or cell replacement therapies.

【 授权许可】

CC BY