Caffeine and acetaminophen association: Effects on mitochondrial bioenergetics

LIFE SCIENCES

卷:193

Article

Goncalves, Debora F.¹  de Carvalho, Nelson R.²  Leite, Martim B.¹  Courtes, Aline A.¹  Hartmann, Diane D.¹  Stefanello, Silvio T.¹  da Silva, Ingrid K.¹  Franco, Jeferson L.^1,2  Soares, Felix A. A.¹  Dalla Corte, Cristiane L.^1,3 

[1] Univ Fed Santa Maria, Ctr Ciencias Nat & Exatas, Dept Bioquim & Biol Mol, Programa Posgrad Ciencias Biol,Bioquim Toxicol, BR-97105900 Santa Maria, RS, Brazil

[2] Univ Fed Pampa, Ctr Interdisciplinar Pesquisa Biotecnol, CIPBIOTEC, Campus Sao Gabriel, BR-97300000 Sao Gabriel, RS, Brazil

[3] Univ Fed Pampa, Campus Cacapava do Sul, BR-96570000 Cacapava Do Sul, RS, Brazil

关键词: Mitochondria;    High-resolution respirometry;    Oxidative stress;    Bioenergetics;   

DOI  :  10.1016/j.lfs.2017.10.039

来源: Elsevier

【 摘 要 】

Aims: Many studies have been demonstrating the role of mitochondrial function in acetaminophen (APAP) hepatotoxicity. Since APAP is commonly consumed with caffeine, this work evaluated the effects of the combination of APAP and caffeine on hepatic mitochondrial bioenergetic function in mice. Main methods: Mice were treated with caffeine (20 mg/kg, intraperitoneal (i.p.)) or its vehicle and, after 30 minutes, APAP (250 mg/kg, i.p.) or its vehicle. Four hours later, livers were removed, and the parameters associated with mitochondrial function and oxidative stress were evaluated. Hepatic cellular oxygen consumption was evaluated by high-resolution respirometry (HRR). Key findings: APAP treatment decreased cellular oxygen consumption and mitochondrial complex activities in the livers of mice. Additionally, treatment with APAP increased swelling of isolated mitochondria from mice livers. On the other hand, caffeine administered with APAP was able to improve hepatic mitochondrial bioenergetic function. Treatment with APAP increased lipid peroxidation and reactive oxygen species (ROS) production and decreased glutathione levels in the livers of mice. Caffeine administered with APAP was able to prevent lipid peroxidation and the ROS production in mice livers, which may be associated with the improvement of mitochondrial function caused by caffeine treatment. Significance: We suggest that the antioxidant effects of caffeine and/or its interactions with mitochondrial bioenergetics may be involved in its beneficial effects against APAP hepatotoxicity.

【 授权许可】

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