Characterization of magnetite nanoparticles for SQUID-relaxometry and magnetic needle biopsy

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:321

Article; Proceedings Paper

Adolphi, Natalie L.¹  Huber, Dale L.²  Jaetao, Jason E.³  Bryant, Howard C.¹  Lovato, Debbie M.³  Fegan, Danielle L.¹  Venturini, Eugene L.²  Monson, Todd C.²  Tessier, Trace E.¹  Hathaway, Helen J.⁴  Bergemann, Christian⁵  Larson, Richard S.³  Flynn, Edward R.¹ 

[1] Senior Sci LLC, Albuquerque, NM 87111 USA

[2] Sandia Natl Labs, Albuquerque, NM 87185 USA

[3] Univ New Mexico, Dept Pathol, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA

[4] Univ New Mexico, Dept Cell Biol & Physiol, Albuquerque, NM 87131 USA

[5] Chemicell GmbH, D-12103 Berlin, Germany

关键词: Magnetite;    Nanoparticle;    Magnetorelaxometry;    SQUID detection;    Susceptometry;    Antibody-conjugation;   

DOI  :  10.1016/j.jmmm.2009.02.067

来源: Elsevier

【 摘 要 】

Magnetite nanoparticles(Chemicell SiMAG-TCL) were characterized by SQUID-relaxometry, susceptometry, and TEM. The magnetization detected by SQUID-relaxometry was 0.33% of that detected by susceptometry, indicating that the sensitivity of SQUID-relaxometry could be significantly increased through improved control of nanoparticle size. The relaxometry data were analyzed by the moment superposition model(MSM) to determine the distribution of nanoparticle moments. Analysis of the binding of CD34-conjugated nanoparticles to U937 leukemia cells revealed 60,000 nanoparticles per cell, which were collected from whole blood using a prototype magnetic biopsy needle, with a capture efficiency of >65% from a 750 mu l sample volume in 1 min. (c) 2009 Elsevier B.V. All rights reserved.

【 授权许可】

Free