Multifunctional PEG-carboxylate copolymer coated superparamagnetic iron oxide nanoparticles for biomedical application

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:451

Article

Illes, Erzsebet¹  Szekeres, Marta¹  Toth, Ildiko Y.¹  Szabo, Akos²  Ivan, Bela²  Turcu, Rodica³  Vekas, Ladislau⁴  Zupko, Istvan⁵  Jaics, Gyorgy¹  Tombacz, Etelka¹ 

[1] Univ Szeged, Dept Phys Chem & Mat Sci, Aradi Vt 1, H-6720 Szeged, Hungary

[2] Hungarian Acad Sci, Res Ctr Nat Sci, Inst Mat & Environm Chem, Polymer Chem Res Grp, POB 286, H-1519 Budapest, Hungary

[3] Natl Inst R&D Isotop & Mol Technol, 67-103 Donat Str, RO-400293 Cluj Napoca, Romania

[4] Ctr Fundamental & Adv Tech Res, Mihai Viteazul Str 24, Timisoara 300223, Romania

[5] Univ Szeged, Dept Pharmacodynam & Biopharm, Eotvos U 1, H-6720 Szeged, Hungary

关键词: Core-shell nanoparticles;    Superparamagnetic iron oxide nanoparticles (SPIONs);    PEGylation;    Colloidal stability;    Cell internalization;    Theranostics;   

DOI  :  10.1016/j.jmmm.2017.11.122

来源: Elsevier

【 摘 要 】

Biocompatible magnetite nanoparticles (MNPs) were prepared by post-coating the magnetic nanocores with a synthetic polymer designed specifically to shield the particles from non-specific interaction with cells. Poly(ethylene glycol) methyl ether methacrylate (PEGMA) macromonomers and acrylic acid (AA) small molecular monomers were chemically coupled by quasi-living atom transfer radical polymerization (ATRP) to a comb-like copolymer, P(PEGMA-co-AA) designated here as P(PEGMA-AA). The polymer contains pendant carboxylate moieties near the backbone and PEG side chains. It is able to bind spontaneously to MNPs; stabilize the particles electrostatically via the carboxylate moieties and sterically via the PEG moieties; provide high protein repellency via the structured PEG layer; and anchor bioactive proteins via peptide bond formation with the free carboxylate groups. The presence of the P(PEGMA-AA) coating was verified in XPS experiments. The electrosteric (i.e., combined electrostatic and steric) stabilization is efficient down to pH 4 (at 10 mM ionic strength). Static magnetization and AC susceptibility measurements showed that the P(PEGMA-AA)@MNPs are superparamagnetic with a saturation magnetization value of 55 emu/g and that both single core nanoparticles and multicore structures are present in the samples. The multicore components make our product well suited for magnetic hyperthermia applications (SAR values up to 17.44 W/g). In vitro biocompatibility, cell internalization, and magnetic hyperthermia studies demonstrate the excellent theranostic potential of our product. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

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