Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge

Journal of Nanobiotechnology

Research

Feng Zhang¹  Wolfgang J Parak¹  Raimo Hartmann¹  Pilar Rivera_Gil²  Thomas H Kissel³  Christoph Schweiger³ 

[1] Biophotonics Group and WZMW, Institute of Physics, Philipps University of Marburg, Renthof 7, D 35037, Marburg, Germany;Biophotonics Group and WZMW, Institute of Physics, Philipps University of Marburg, Renthof 7, D 35037, Marburg, Germany;Biophotonics Group and WZMW, Institute of Physics, Philipps University of Marburg, Renthof 7, D 35037, Marburg, Germany;Pharmaceutics and Biopharmacy, Faculty of Pharmacy, Philipps University of Marburg, Ketzerbach 63, D 35037, Marburg, Germany;

关键词: Superparamagnetic iron oxide nanoparticles (SPIONs);    Intracellular distribution;    Charge;    Coating;    Size;    Quantitative correlation analysis;    Colocalization;   

DOI  :  10.1186/1477-3155-10-28

 received in 2012-04-02, accepted in 2012-05-28,  发布年份 2012

来源: Springer

【 摘 要 】

Time-resolved quantitative colocalization analysis is a method based on confocal fluorescence microscopy allowing for a sophisticated characterization of nanomaterials with respect to their intracellular trafficking. This technique was applied to relate the internalization patterns of nanoparticles i.e. superparamagnetic iron oxide nanoparticles with distinct physicochemical characteristics with their uptake mechanism, rate and intracellular fate.The physicochemical characterization of the nanoparticles showed particles of approximately the same size and shape as well as similar magnetic properties, only differing in charge due to different surface coatings. Incubation of the cells with both nanoparticles resulted in strong differences in the internalization rate and in the intracellular localization depending on the charge. Quantitative and qualitative analysis of nanoparticles-organelle colocalization experiments revealed that positively charged particles were found to enter the cells faster using different endocytotic pathways than their negative counterparts. Nevertheless, both nanoparticles species were finally enriched inside lysosomal structures and their efficiency in agarose phantom relaxometry experiments was very similar.This quantitative analysis demonstrates that charge is a key factor influencing the nanoparticle-cell interactions, specially their intracellular accumulation. Despite differences in their physicochemical properties and intracellular distribution, the efficiencies of both nanoparticles as MRI agents were not significantly different.

【 授权许可】

Unknown   
© Schweiger et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.