Yogic meditation reverses NF-κB and IRF-related transcriptome dynamics in leukocytes of family dementia caregivers in a randomized controlled trial

PSYCHONEUROENDOCRINOLOGY

卷:38

Article

Black, David S.¹  Cole, Steve W.^1,2,3  Irwin, Michael R.^1,2  Breen, Elizabeth^1,2  St Cyr, Natalie M.²  Nazarian, Nora²  Khalsa, Dharma S.⁴  Lavretsky, Helen² 

[1] Univ Calif Los Angeles, Semel Inst Neurosci & Human Behav, Cousins Ctr Psychoneuroimmunol, Los Angeles, CA USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Sch Med, Div Hematol Oncol, Dept Med, Los Angeles, CA 90095 USA

[4] Alzheimers Res & Prevent Fdn, Tucson, AZ 85715 USA

关键词: Dementia caregiver;    Stress;    Kirtan Kriya;    Yoga;    Meditation;    Relaxation;    NF-kappa B;    Genomics;    Gene expression profiling;    Microarray;   

DOI  :  10.1016/j.psyneuen.2012.06.011

来源: Elsevier

【 摘 要 】

Background: Although yoga and meditation have been used for stress reduction with reported improvement in inflammation, little is known about the biological mechanisms mediating such effects. The present study examined if a yogic meditation might alter the activity of inflammatory and antiviral transcription control pathways that shape immune cell gene expression. Methods: Forty-five family dementia caregivers were randomized to either Kirtan Kriya Meditation (KKM) or Relaxing Music (RM) listening for 12 min daily for 8 weeks and 39 caregivers completed the study. Genome-wide transcriptional profiles were collected from peripheral blood leukocytes sampled at baseline and 8-week follow-up. Promoter-based bioinformatics analyses tested the hypothesis that observed transcriptional alterations were structured by reduced activity of the pro-inflammatory nuclear factor (NF)-kappa B family of transcription factors and increased activity of Interferon Response Factors (IRFs; i.e., reversal of patterns previously linked to stress). Results: In response to KKM treatment, 68 genes were found to be differentially expressed (19 up-regulated, 49 down-regulated) after adjusting for potentially confounded differences in sex, illness burden, and BMI. Up-regulated genes included immunoglobulin-related transcripts. Down-regulated transcripts included pro-inflammatory cytokines and activation-related immediate-early genes. Transcript origin analyses identified plasmacytoid dendritic cells and B lymphocytes as the primary cellular context of these transcriptional alterations (both p < .001). Promoter-based bioinformatic analysis implicated reduced NF-kappa B signaling and increased activity of IRF1 in structuring those effects (both p < .05). Conclusion: A brief daily yogic meditation intervention may reverse the pattern of increased NF-kappa B-related transcription of pro-inflammatory cytokines and decreased IRF1-related transcription of innate antiviral response genes previously observed in healthy individuals confronting a significant life stressor. (C) 2012 Elsevier Ltd. All rights reserved.

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