Blood-based gene-expression biomarkers of post-traumatic stress disorder among deployed marines: A pilot study

PSYCHONEUROENDOCRINOLOGY

卷:51

Article

Tylee, Daniel S.^1,2  Chandler, Sharon D.³  Nievergelt, Caroline M.^4,5,6  Liu, Xiaohua^3,10  Pazol, Joel³  Woelk, Christopher H.^5,7  Lohr, James B.^4,5,6  Kremen, William S.^3,4,6  Baker, Dewleen G.^4,5,6  Glatt, Stephen J.^1,2  Tsuang, Ming T.^3,4,5,6,8,9 

[1] SUNY Upstate Med Univ, Psychiat Genet Epidemiol & Neurobiol Lab PsychGEN, Dept Psychiat & Behav Sci, Med Genet Res Ctr, Syracuse, NY 13210 USA

[2] SUNY Upstate Med Univ, Med Genet Res Ctr, Dept Neurosci & Physiol, Syracuse, NY 13210 USA

[3] Univ Calif San Diego, Dept Psychiat, Ctr Behav Genom, San Diego, CA 92103 USA

[4] VA Ctr Excellence Stress & Mental Hlth, San Diego, CA USA

[5] Vet Affairs San Diego Healthcare Syst, San Diego, CA USA

[6] Univ Calif San Diego, Dept Psychiat, San Diego, CA 92103 USA

[7] Univ Calif San Diego, Dept Med, San Diego, CA 92103 USA

[8] Univ Calif San Diego, Inst Genom Med, San Diego, CA 92103 USA

[9] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Harvard Inst Psychiat Epidemiol & Genet, Boston, MA 02115 USA

[10] Shanghai Jiao Tong Univ, Sch Med, Shanghai Mental Hlth Ctr, Shanghai 200030, Peoples R China

关键词: Alternative splicing;    mRNA;    Peripheral blood mononuclear cells;    Microarray;    Transcriptome;    Trauma;    Diagnosis;    Biomarker;    Antioxidant;    Oxidative stress;   

DOI  :  10.1016/j.psyneuen.2014.09.024

来源: Elsevier

【 摘 要 】

The etiology of post-traumatic stress disorder (PTSD) likely involves the interaction of numerous genes and environmental factors. Similarly, gene-expression levels in peripheral blood are influenced by both genes and environment, and expression levels of many genes show good correspondence between peripheral blood and brain tissues. In that context, this pilot study sought to test the following hypotheses: (1) post-trauma expression levels of a gene subset in peripheral blood would differ between Marines with and without PTSD; (2) a diagnostic biomarker panel of PTSD among high-risk individuals could be developed based on gene-expression in readily assessable peripheral blood cells; and (3) a diagnostic panel based on expression of individual exons would surpass the accuracy of a model based on expression of full-length gene transcripts. Gene-expression levels in peripheral blood samples from 50 U.S. Marines (25 PTSD cases and 25 non-PTSD comparison subjects) were determined by microarray following their return from deployment to war-zones in Iraq or Afghanistan. The original sample was carved into training and test subsets for construction of support vector machine classifiers. The panel of peripheral blood biomarkers achieved 80% prediction accuracy in the test subset based on the expression of just two full-length transcripts (GSTM1 and GSTM2). A biomarker panel based on 20 exons attained an improved 90% accuracy in the test subset. Though further refinement and replication of these biomarker profiles are required, these preliminary results provide proof-of-principle for the diagnostic utility of blood-based mRNA-expression in PTSD among trauma-exposed individuals. (C) 2014 Elsevier Ltd. All rights reserved.

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