Involvement of the p62/Nrf2/HO-1 pathway in the browning effect of irisin in 3T3-L1 adipocytes

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:514

Article

Tsai, Yung-Chieh^1,2,3  Wang, Chyi-Wen⁴  Wen, Bo-Yao⁴  Hsieh, Po-Shiuan⁴  Lee, Yen-Mei⁵  Yen, Mao-Hsiung⁵  Cheng, Pao-Yun⁴ 

[1] Chi Mei Med Ctr, Dept Obstet & Gynecol, Tainan, Taiwan

[2] Taipei Med Univ, Dept Med, Taipei, Taiwan

[3] Chia Nan Univ Pharm & Sci, Dept Sport Management, Tainan, Taiwan

[4] Natl Def Med Ctr, Grad Inst Physiol, Dept Physiol & Biophys, 161,Sec 6,Minquan East Rd, Taipei 114, Taiwan

[5] Natl Def Med Ctr, Dept Pharmacol, Taipei, Taiwan

关键词: Irisin;    Browning;    p62;    Haeme oxygenase-1;    Adipocyte;   

DOI  :  10.1016/j.mce.2020.110915

来源: Elsevier

【 摘 要 】

Irisin has gained attention because of its potential applications in the treatment of metabolic diseases. Accumulating evidence indicates that irisin attenuates obesity via the browning of white adipose tissue; however, the underlying mechanisms are unclear. Here, we evaluated the effects of irisin on adipocyte browning and the underlying mechanisms. The western blotting and immunofluorescence analyses demonstrated that irisin significantly induced the up-regulation of brown fat-specific proteins (PGC1 alpha, PRDM16, and UCP-1) and HO-1 in 3T3-L1 adipocytes. Moreover, irisin significantly increased the levels of cytosolic p62 and nuclear Nrf2. These effects of irisin in the adipocytes were attenuated by treatment with SnPP or p62 siRNA. In addition, the browning effect of irisin was observed in BAT-WT-1 cells. These findings suggest that irisin induced browning effect via the p62/Nrf2/HO-1 signalling pathway and that it may be a potential candidate for preventing or treating obesity.

【 授权许可】

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