NaClO effect on polyphenol oxidase activity in leaf and petiole explants of two of Jatropha curcas L. genotypes

Bioagro

Aguirre-Arzola, Víctor¹  Ojeda-Zacarías, María¹  Universidad Autónoma de Nuevo León, Escobedo, México¹  Rodríguez-Pérez, Gilberto¹  Universidad Autónoma de Nuevo León, Marín, México¹  Pequeño-Granado, Iván¹  Universidad Juárez del Estado de Durango, Durango, México¹  Mojica-Marín, Virgilio¹  Instituto Tecnológico de Roque, Roque Celaya, México¹  Instituto Nacional Investigaciones Forestales, Agrícolas y Pecuarias, Rosario Izapa, México¹  Martínez-Ávila, Guillermo¹  Iracheta-Donjuan, Leobardo¹ 

关键词: Browning;    in vitro;    PPO;    surface disinfection;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidad Centroccidental Lisandro Alvarado * Decanato de Agronomia

【 摘 要 】

The implementation of plant tissue culture of Jatropha curcas encounters many problems such as the browning caused by phenolic oxidation. Therefore, a rapid methodology for comparison of treatments that can prevent this oxidation of the plant tissue is required. The determination of the enzyme polyphenol oxidase (PPO) activity could be used for this purpose due to the association between browning and the activity of the enzyme in J. curcas. In the present investigation the effect of sodium hypochlorite (NaClO) as a disinfecting agent on PPO enzyme activity in leaf and petiole explants of INI-3 and INI-6 genotypes of the species was evaluated. The results showed that the NaClO caused an increase in the PPO activity of leaves and petioles in both genotypes, thus indicating that the disinfectant is an indirect agent that can promote tissue browning in the plant. In addition, significant differences of PPO activity were found in both explant types and genotypes.

【 授权许可】

Unknown