Targeted RNA sequencing of adrenal zones using immunohistochemistry-guided capture of formalin-fixed paraffin-embedded tissue

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:530

Article

Baker, Jessica E.¹  Plaska, Samuel W.¹  Qin, Zhaoping²  Liu, Chia-Jen³  Rege, Juilee¹  Rainey, William E.^1,5,6  Udager, Aaron M.^3,4,5 

[1] Univ Michigan, Med Sch, Dept Mol & Integrat Physiol, 2558 Med Sci Res Bldg 2,1150 W Med Ctr Dr, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Med Sch, Dept Internal Med, Div Hematol Oncol, Ann Arbor, MI 48109 USA

[3] Michigan Ctr Translat Pathol, Ann Arbor, MI USA

[4] Univ Michigan, Med Sch, Dept Pathol, 3308 Rogel Canc Ctr,1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Rogel Canc Ctr, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Med Sch, Dept Internal Med, Div Metab Endocrine & Diabet, Ann Arbor, MI 48109 USA

关键词: RNAseq;    Adrenal cortex;    Zona glomerulosa;    Zona fasciculata;    Zona reticularis;    Adrenal medulla;   

DOI  :  10.1016/j.mce.2021.111296

来源: Elsevier

【 摘 要 】

Adequate access to fresh or frozen normal adrenal tissue has been a primary limitation to the enhanced characterization of the adrenal zones via RNA sequencing (RNAseq). Herein, we describe the application of targeted RNAseq to formalin-fixed paraffin-embedded (FFPE) normal adrenal gland specimens. Immunohistochemistry (IHC) was used to visualize and guide the capture of the adrenocortical zones and medulla. Following IHC-based tissue capture and isolation of RNA, high-throughput targeted RNAseq highlighted clear transcriptomic differences and identified differentially expressed genes among the adrenal zones. Our data demonstrate the ability to capture FFPE adrenal zone tissue for targeted transcriptomic analyses. Future comparison of normal adrenal zones will improve our understanding of transcriptomic patterns and help identify potential novel pathways controlling zone-specific steroid production.

【 授权许可】

Free