Damaged spermatogenic cells induce inflammatory gene expression in mouse Sertoli cells through the activation of Toll-like receptors 2 and 4

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:365

Article

Zhang, Xiaoyan¹  Wang, Tao¹  Deng, Tingting¹  Xiong, Weipeng¹  Lui, Peng¹  Li, Nan¹  Chen, Yongmei¹  Han, Daishu¹ 

[1] Chinese Acad Med Sci, Sch Basic Med, Peking Union Med Coll, Dept Cell Biol,Inst Basic Med Sci, Beijing 100730, Peoples R China

关键词: Sertoli cell;    Toll-like receptor;    Testis;    Inflammation;   

DOI  :  10.1016/j.mce.2012.10.016

来源: Elsevier

【 摘 要 】

Testicular inflammation, including noninfectious inflammatory responses in the testis, may impair male fertility. Mechanisms underlying the initiation of noninfectious testicular inflammation are poorly understood. In the current study, we demonstrate that damaged spermatogenic cell products (DSCPs) induce expression of various inflammatory mediators, including TNF-alpha, IL-1 beta, IL-6, and macrophage chemotactic protein 1 (MCP-1), in Sertoli cells. Notably, the DSCP-induced inflammatory gene expression was significantly reduced by knockout Toll-like receptor (TLR)2 or TLR4, and abolished by double knockout TLR2 and TLR4 (TLR2(-/-)TLR4(-/-)). MCP-1 secreted by Sertoli cells after stimulation with DSCPs promotes macrophage migration. We also provide evidence that busulfan-induced spermatogenic cell damages in vivo upregulate TNF-alpha and MCP-1 expression in Sertoli cells, and facilitate macrophage infiltration into the testis in wild-type mice. These phenomena were not observed in TLR2(-/-)TLR4(-/-) mice. Data indicate that DSCPs induce inflammatory gene expression in Sertoli cells via the activation of TLR2 and TLR4, which may initiate noninfectious inflammatory responses in the testis. The results provide novel insights into the mechanisms underlying damaged spermatogenic cell-induced testicular inflammation. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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