Localization of hormone‐sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes

FEBS Letters

Bergh, Anders²  Holst, Lena Stenson³  Mulder, Hindrik¹  Fredrikson, Gudrun³  Holm, Cecilia³  Hoffmann, Anna Maria²  Sundler, Frank¹ 

[1]Department of Medical Cell Research, Lund University, Lund, Sweden

[2]Departments of Pathology and Medical Chemistry and Biophysics, University of Umeå, Umeå, Sweden

[3]Department of Medical and Physiological Chemistry, Section for Molecular Signalling, Lund University, PO Box 94, S-221 00 Lund, Sweden

关键词: Hormone sensitive lipase;    Cholesterol ester hydrolase;    Testis;    Sertoli cell;    In situ hybridization;    EDS;    ethane dimethane sulphonate;    DFP;    diisopropyl fluorophosphate;    HSL;    hormone-sensitive lipase;    SDS-PAGE;    polyacrylamide gel-electrophoresis in sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(94)01185-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.

【 授权许可】

Unknown