Identification and characterization of estrogen receptor-related receptor alpha and gamma in human glioma and astrocytoma cells

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:315

Article

Gandhari, Mukesh K.¹  Frazier, Chester R.¹  Hartenstein, Julia S.¹  Cloix, Jean-Francois²  Bernier, Michel¹  Wainer, Irving W.¹ 

[1] NIA, Clin Invest Lab, Biomed Res Ctr, NIH, Baltimore, MD 21224 USA

[2] Univ Orleans, Neurobiol Lab, F-45067 Orleans 2, France

关键词: DY131;    Biochanin A;    XCT790;    4-Hydroxytamoxifen;    AAB-TATA-Luc;    3xERE-TATA-Luc;    T98G;    U87MG;    A-172 cells;    ERR gamma;    ERR alpha;   

DOI  :  10.1016/j.mce.2009.10.001

来源: Elsevier

【 摘 要 】

The purpose of this study was to examine expression and function of estrogen receptor-related receptors (ERRs) in human glioma and astrocytoma cell lines. These estrogen receptor-negative cell lines expressed ERR alpha and ERR gamma proteins to varying degree in a cell context dependent manner, with U87MG glioma cells expressing both orphan nuclear receptors. Cell proliferation assays were performed in the presence of ERR isoform-specific agonists and antagonists, and the calculated EC50 and IC50 values were consistent with previous reported values determined in other types of cancer cell lines. Induction of luciferase expression under the control of ERR isoform-specific promoters was also observed in these cells. These results indicate that ERR alpha and ERR gamma are differentially expressed in these tumor cell lines and likely contribute to agonist-dependent ERR transcriptional activity. Published by Elsevier Ireland Ltd.

【 授权许可】

Free