Messenger RNA profile analysis deciphers new Esrrb responsive genes in prostate cancer cells

BMC Molecular Biology

Research Article

Dennis B. Lubahn¹  Yuan Lu²  Jilong Li³  Jianlin Cheng³ 

[1] Department of Biochemistry, University of Missouri, 65211, Columbia, MO, USA;MU Center for Botanical Interaction Studies, University of Missouri, 65211, Columbia, MO, USA;Department of Biochemistry, University of Missouri, 65211, Columbia, MO, USA;MU Center for Botanical Interaction Studies, University of Missouri, 65211, Columbia, MO, USA;Xiphophorus Genetic Stock Center, Texas State University, 78666, San Marcos, TX, USA;MU Center for Botanical Interaction Studies, University of Missouri, 65211, Columbia, MO, USA;Computer Science Department, University of Missouri, 65211, Columbia, MO, USA;Informatics Institute, University of Missouri, 65211, Columbia, MO, USA;

关键词: Estrogen related receptor;    RNA-Seq;    Prostate cancer;    Gene expression profile;    DY131;    Transcription regulation;    Nuclear receptor;   

DOI  :  10.1186/s12867-015-0049-1

 received in 2015-06-28, accepted in 2015-11-13,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundOrphan nuclear receptor estrogen related receptor β (Esrrb or ERRβ) is well known in stem cells and early embryonic development. However, little is known about its function in cancer.MethodWe investigated the mRNA profile alterations induced by Esrrb expression and its synthetic ligand DY131 in human prostate cancer DU145 cells via RNA-Seq analysis.ResultsWe distinguished 67 mRNAs differentially expressed by Esrrb alone. Although DY131 alone did not change any gene, treatment of DY131 in the presence of Esrrb altered 1161 mRNAs. These observations indicated Esrrb had both ligand-independent and ligand-dependent activity. When Esrrb was expressed, DY131 treatment further regulated 15 Esrrb-altered mRNAs. DY131 acted as an antagonist for 11 of 15 mRNAs (wdr52, f13a1, pxdn, spns2, loc100506599, tagln, loc441454, tkel1, sema3f, zcwpw2, sdc2) and as an agonist for 4 of the 15 mRNAs (rarres3, oasl, padi2, ddx60). Gene ontology analyses showed altered genes are related to transcription and translation regulation, cell proliferation and apoptosis regulation, and cellular metabolism.ConclusionOur results characterized mRNA profiles in DU145 prostate cancer cells driven by Esrrb expression and Esrrb ligand DY131, and provided multiple markers to characterize Esrrb’s function in Esrrb research.

【 授权许可】

CC BY   
© Lu et al. 2015