Genes targeted by the Hedgehog-signaling pathway can be regulated by Estrogen related receptor β

BMC Molecular Biology

Dennis B. Lubahn²  Jianlin Cheng³  Jilong Li³  Yuan Lu¹ 

[1] Xiphophorus Genetic Stock Center, Texas State University, San Marcos 78666, TX, USA;MU Center for Botanical Interaction Studies, University of Missouri, Columbia 65211, MO, USA;Informatics Institute, University of Missouri, Columbia 65211, MO, USA

关键词: Gene interactions;    RNA-Seq;    Gene expression profiling;    Hedgehog signaling pathway;    Estrogen related receptor;   

Others  :  1234463
DOI  :  10.1186/s12867-015-0047-3

 received in 2015-07-02, accepted in 2015-11-06,  发布年份 2015

【 摘 要 】

Background

Nuclear receptor family member, Estrogen related receptor β, and the Hedgehog signal transduction pathway are both reported to relate to tumorigenesis and induced pluripotent stem cell reprogramming. We hypothesize that Estrogen related receptor β can modulate the Hedgehog signaling pathway and affect Hedgehog driven downstream gene expression.

Results

We established an estrogen related receptor β-expressing Hedgehog-responsive NIH3T3 cell line by Esrrb transfection, and performed mRNA profiling using RNA-Seq after Hedgehog ligand conditioned medium treatment. Esrrb expression altered 171 genes, while Hedgehog signaling activation alone altered 339 genes. Additionally, estrogen related receptor β expression in combination with Hedgehog signaling activation affects a group of 109 Hedgehog responsive mRNAs, including Hsd11b1, Ogn, Smoc2, Igf1, Pdcd4, Igfbp4, Stmn1, Hp, Hoxd8, Top2a, Tubb4b, Sfrp2, Saa3, Prl2c3 and Dpt.

Conclusions

We conclude that Estrogen related receptor β is capable of interacting with Hh-signaling downstream targets. Our results suggest a new level of regulation of Hedgehog signaling by Estrogen related receptor β, and indicate modulation of Estrogen related receptor β can be a new strategy to regulate various functions driven by the Hedgehog signaling pathway.

【 授权许可】

   
2015 Lu et al.

【 图 表 】

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