Cooperativity between 11β-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase is based on a common pyridine nucleotide pool in the lumen of the endoplasmic reticulurn

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:248

Article; Proceedings Paper

Czegle, I ; Piccirella, S ; Senesi, S ; Csala, M ; Mandl, J ; Bánhegyi, G ; Fulceri, R ; Benedetti, A

关键词: 11 beta-hydroxysteroid dehydrogenase type 1;    hexose-6-phosphate dehydrogenase;    pyridine nucleotides;    rat liver microsomes;   

DOI  :  10.1016/j.mce.2005.10.018

来源: Elsevier

【 摘 要 】

11 beta-Hydroxysteroid dehydrogenase type 1 (11 beta HSD1) is a NADP(H)-dependent oxidoreductase of the ER lumen, which may have an important role in the pathogenesis of metabolic syndrome. Here, the functional coupling of 11 beta-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase (H6PDH) was investigated in rat liver microsomal vesicles. The results demonstrate the existence of a separate intraluminal pyridine nucleotide pool in the hepatic endoplasmic reticulum and a close cooperation between 11 beta PHSD1 and H6PDH based on their co-localization and the mutual generation of cofactors for each other. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free