In Vitro Glucuronidation of Ochratoxin A by Rat Liver Microsomes

Toxins

Zheng Han¹  Emmanuel K. Tangni²  José Diana Di Mavungu⁴  Lynn Vanhaecke³  Sarah De Saeger⁴  Aibo Wu¹ 

[1] Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, China; E-Mail:;Veterinary and Agrochemical Research Centre (CODA-CERVA), Unit of Toxins and Natural Components, Leuvensesteenweg 17, Tervuren B-3080, Belgium; E-Mails:;Laboratory of Chemical Analysis, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke B-9820, Belgium; E-Mail:;Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, Ghent B-9000, Belgium; E-Mails:

关键词: ochratoxin A;    glucuronidation;    metabolic pathway;    rat liver microsomes;    Orbitrap;   

DOI  :  10.3390/toxins5122671

来源: mdpi

【 摘 要 】

Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and liquid chromatography-multiple stage mass spectrometry (LC-MSⁿ) were utilized to investigate the metabolic profile of OTA in rat liver microsomes. Three conjugated products of OTA corresponding to amino-, phenol- and acyl-glucuronides were identified, and the related structures were confirmed by hydrolysis with β-glucuronidase. Moreover, OTA methyl ester, OTα and OTα-glucuronide were also found in the reaction solution. Based on these results, an in vitro metabolic pathway of OTA has been proposed for the first time.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.