Human granulosa cells function as innate immune cells executing an inflammatory reaction during ovulation: a microarray analysis

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:486

Article

Poulsen, Liv la Cour¹  Englund, Anne Lis Mikkelsen¹  Wissing, Marie Louise Muff¹  Andersen, Claus Yding²  Borup, Rehannah³  Grondahl, Marie Louise⁴ 

[1] Zealand Univ Hosp, Zealand Fertil Clin, Lykkebaekvej 14, DK-4600 Koge, Denmark

[2] Univ Hosp Copenhagen, Lab Reprod Biol, Rigshosp, Blegdamsvej 9, DK-92100 Copenhagen O, Denmark

[3] Univ Copenhagen, Fac Hlth & Med Sci, Ctr Chromosome Stabil, Dept Cellular & Mol Med, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark

[4] Univ Hosp Copenhagen, Herlev & Gentofte Hosp, Herlev Fertil Clin, Herlev Ringvej 75, DK-2730 Herlev, Denmark

关键词: Ovulation;    Inflammatory response;    Innate immunity;    Granulosa cells;    Microarray;    LH;   

DOI  :  10.1016/j.mce.2019.02.014

来源: Elsevier

【 摘 要 】

Ovulation has been compared to a local inflammatory reaction. We performed an in silico study on a unique, PCR validated, transcriptome microarray study to evaluate if known inflammatory mechanisms operate during ovulation. The granulosa cells were obtained in paired samples at two different time points during ovulation (just before and 36 hours after ovulation induction) from nine women receiving fertility treatment. A total of 259 genes related to inflammation became significantly upregulated during ovulation (2-80 fold, p < 0.05), while specific leukocyte markers were absent. The genes and pathway analysis indicated NF-KB-, MAPK- and JAK/STAT signalling (p < 1.0E-10) as the major pathways involved in danger recognition and cytokine signalling to initiate inflammation. Upregulated genes further encoded enzymes in eicosanoid production, chemo-attractants, coagulation factors, cell proliferation factors involved in tissue repair, and anti-inflammatory factors to resolve the inflammation again. We conclude that granulosa cells, without involvement from the innate immune system, can orchestrate ovulation as a complete sterile inflammatory reaction.

【 授权许可】

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