A second gene encodes the anti-diuretic hormone in the insect, Rhodnius prolixus

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:317

Article

Paluzzi, Jean-Paul¹  Orchard, Ian¹ 

[1] Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada

关键词: CAPA;    CAP2b;    Anti-diuresis;    Peptide;    Hormone;    Insect;   

DOI  :  10.1016/j.mce.2009.11.004

来源: Elsevier

【 摘 要 】

In the haematophagous insect, Rhodnius prolixus, a rapid diuresis following engorgement of vertebrate blood is under the control of two main diuretic hormones: a corticotropin-releasing factor (CRF)-related peptide andserotonin (5HT). A CAP2b (CAPA)-related peptide is involved in the termination of this diuresis, and we have recently identified a gene, now referred to as RhoprCAPA-alpha, encoding CAPA peptides in R. prolixus. Here we identify a second gene, RhoprCAPA-beta, which also encodes CAPA peptides and characterize its expression in fifth-instar and adults. The RhoprCAPA-beta gene is more highly expressed in the CNS than the RhoprCAPA-alpha gene, but neither gene is expressed in other tested adult tissues. Both genes are expressed in a subset of immunoreactive neurons identified using an antisera which recognizes CAP2b-related peptides. The expression of each paralog is modified by feeding and we propose this to be a result of requirements of anti-diuretic regulation during salt and water homeostasis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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