Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection

THIN SOLID FILMS

卷:519

Article; Proceedings Paper

Sharma, Aditya¹  Matharu, Zimple¹  Sumana, G.¹  Solanki, Pratima R.¹  Kim, C. G.²  Malhotra, B. D.^1,2 

[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, Biomed Instrumentat Sect,Mat Phys & Engn Div,Coun, New Delhi 110012, India

[2] Chungnam Natl Univ, Ctr NanoBioEngn & Spintron, Taejon 305764, South Korea

关键词: Aflatoxin;    Cysteamine;    Gold nanoparticles;    Immunosensor;    Self assembled monolayer;   

DOI  :  10.1016/j.tsf.2010.08.071

来源: Elsevier

【 摘 要 】

Aflatoxin B-1 antibody (aAFB(1)) covalently attached to cysteamine functionalized-gold nanoparticles (C-AuNP) has been immobilized onto 4-mercaptobenzoic acid (MBA) based self assembled monolayer (SAM) on gold electrode (MBA/Au), for the fabrication of BSA/aAFB(1)-C-AuNP/MBA/Au immunoelectrode. This immunoelectrode has been characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and electrochemical characterization techniques. The electrochemical response studies reveal that the BSA/aAFB(1)-C-AuNP/MBA/Au immunoelectrode can be used to detect AFBI in the range of 10-100 ng dL(-1) and has sensitivity as 0.45 mu A ng(-1)dL, limit of detection as 17.90 ng dL(-1) and a response time of 60 s. (C) 2010 Elsevier B.V. All rights reserved.

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