期刊论文详细信息
TALANTA 卷:194
In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection
Article
Silva, Nadia F. D.1,3  Magalhaes, Jidia M. C. S.2  Fatima Barroso, M.1  Oliva-Teles, Teresa1  Freire, Cristina3  Delerue-Matos, Cristina1 
[1] Inst Politecn Porto, Inst Super Engn Porto, REQUIMTE LAQV, P-4200072 Porto, Portugal
[2] Univ Porto, Fac Engn, Dept Engn Quim, REQUIMTE LAQV, P-4200465 Porto, Portugal
[3] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE LAQV, P-4169007 Porto, Portugal
关键词: Potentiometry;    Gold nanoparticles;    Polymer inclusion membranes;    Immunosensor;    Salmonella typhimurium;   
DOI  :  10.1016/j.talanta.2018.10.024
来源: Elsevier
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【 摘 要 】

Polymeric ion selective electrodes are highly sensitive to changes in zero current ion flow and this offers a route to signal amplification in label-free potentiometric immunosensors. In this work, a label-free potentiometric immunosensor toward Salmonella typhimurium (ST) assembled in a home-made pipette-tip electrode is described. The signal-output amplification was implemented on a gold nanoparticle polymer inclusion membrane (AuNPs-PIM) which was used as sensing platform and for antibody immobilization. Additionally, a marker ion was used to detect the antibody-antigen binding event at the electrode surface. The immunosensor construction was performed in several steps: i) gold salt ions extraction in PVC membrane; ii) AuNPs formation using Na(2)EDTA as reduction agent; iii) antibody anti-Salmonella conjugation on AuNPs-PIM in pipette-tip electrodes. The potential shift observed in potentiometric measurements was derived simply from the blocking effect in the ionic flux caused by antigen-antibody conjugation, without no extra steps, mimetizing the ion-channel sensors. A detection limit of 6 cells mL(-1) was attained. As proof-of-concept, recovery studies were performed in spiked commercial apple juice samples with success. Due to the simplicity of use, the appealing cost of equipment and sensor production and being able to provide a quick analytical response (less than 1 h for a complete assay, including sample preparation for analysis), this scheme represents a good prototype device for the detection of foodborne pathogens like ST or other immune-responsive bacteria.

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