Non-thermal atmospheric pressure plasma jet applied to inactivation of different microorganisms

SURFACE & COATINGS TECHNOLOGY

卷:312

Article; Proceedings Paper

Nishime, T. M. C.¹  Borges, A. C.²  Koga-Ito, C. Y.²  Machida, M.³  Hein, L. R. O.¹  Kostov, K. G.¹ 

[1] Sao Paulo State Univ, UNESP, Campus Guaratingueta FEG, BR-12516410 Guaratingueta, SP, Brazil

[2] Sao Paulo State Univ, UNESP, Inst Sci & Technol ICT, BR-12247004 Sao Jose Dos Campos, SP, Brazil

[3] Univ Estadual Campinas, UNICAMP, Phys Inst IFGW, BR-13083859 Campinas, SP, Brazil

关键词: Plasma jet;    Cold atmospheric plasma;    Decontamination;   

DOI  :  10.1016/j.surfcoat.2016.07.076

来源: Elsevier

【 摘 要 】

Non-thermal atmospheric pressure,plasma jets (APPJs) are capable of generating cold plasma plumes that are not confined by electrodes, which makes them very attractive for bio-medical applications. In the present work, the inactivation efficiency of cold APPJ was evaluated against three pathogenic microorganisms with different cell wall characteristics. The Gram-positive bacterium Enterococcus faecalis (ATCC 29212), the Gram-negative bacterium Pseudomonas aeruginosa (ATCC 15442) and the fungus Candida albicans (SC 5314) were plated on standard Petri dishes filled with specific culture media. The plasma jet with mean power of 1.8 W was directed perpendicularly on agar plates and the system was flushed with pure helium at two different flows, 2.0 and 4.0 SLM. During the treatments, time and distance between nozzle and agar were varied. The presence of excited reactive species was confirmed by optical emission spectroscopy. Scanning electron microscopy (SEM) was applied for investigation of cell morphology. The microbicidal efficiency was evaluated by measuring the area of inhibition zone (where there was no cell growth). For different flows of helium, no significant difference of inhibition zone size was noted for the same microbial species. However, high flows led to formation of non-homogenous inhibition zones, presenting microcolonies distributed through the inactivated region. The Gram-positive bacterium was more susceptible to the plasma antimicrobial effects than the other microorganisms. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

Free