Effect of cold atmospheric plasma treatment on the metabolites of human leukemia cells

Cancer Cell International

Zhijie Liu¹  Xiaohua Wang¹  Dehui Xu¹  Yujing Xu¹  Dingxin Liu¹  Bingchuan Wang¹  Michael G. Kong²  Ning Ning³  Qingjie Cui³  Hailan Chen⁴ 

[1] 0000 0001 0599 1243, grid.43169.39, State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, 710049, Xi’an, Shaanxi, People’s Republic of China;0000 0001 0599 1243, grid.43169.39, State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, 710049, Xi’an, Shaanxi, People’s Republic of China;0000 0001 2164 3177, grid.261368.8, Frank Reidy Center for Bioelectrics, Old Dominion University, 23508, Norfolk, VA, USA;0000 0001 2164 3177, grid.261368.8, Department of Electrical and Computer Engineering, Old Dominion University, 23529, Norfolk, VA, USA;0000 0001 0599 1243, grid.43169.39, The School of Life Science and Technology, Xi’an Jiaotong University, 710049, Xi’an, Shaanxi, People’s Republic of China;0000 0001 2164 3177, grid.261368.8, Frank Reidy Center for Bioelectrics, Old Dominion University, 23508, Norfolk, VA, USA;

关键词: Cold atmospheric plasma;    Acute myeloid leukemia;    Metabolite profiling;    Alanine, aspartate and glutamate metabolism;    -Glutamine and -glutamate metabolism;   

DOI  :  10.1186/s12935-019-0856-4

来源: publisher

【 摘 要 】

BackgroundAcute myeloid leukemia (AML) is a typically fatal malignancy and new drug and treatment need to be developed for a better survival outcome. Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in various of cancer treatment. However, the changes in cell metabolism after CAP treatment of leukemia cells have been rarely studied.MethodsIn this study, we investigated the metabolite profiling of plasma treatment on leukemia cells based on Gas Chromatography Tandem Time-of-Flight Mass Spectrometry (GC-TOFMS). Simultaneously, we conducted a series of bioinformatics analysis of metabolites and metabolic pathways with significant differences after basic data analysis.Results800 signals were detected by GC–TOF mass-spectrometry and then evaluated using PCA and OPLS-DA. All the differential metabolites were listed and the related metabolic pathways were analyzed by KEGG pathway. The results showed that alanine, aspartate and glutamate metabolism had a significant change after plasma treatment. Meanwhile, d-glutamine and d-glutamate metabolism were significantly changed by CAP. Glutaminase activity was decreased after plasma treatment, which might lead to glutamine accumulation and leukemia cells death.ConclusionsWe found the above two metabolic pathways vulnerable to plasma treatment, which might result in leukemia cells death and might be the cornerstone of further exploration of plasma treatment targets.

【 授权许可】

CC BY