D-mannitol sensor based on molecularly imprinted polymer on electrode modified with reduced graphene oxide decorated with gold nanoparticles

TALANTA

卷:165

Article

Beluomini, Maisa Azevedo¹  da Silva, Jose L.¹  Sedenho, Graziela Cristina¹  Stradiotto, Nelson Ramos¹ 

[1] Univ Estadual Paulista UNESP, Inst Chem, Dept Analyt Chem, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP, Brazil

关键词: D-mannitol;    Reduced graphene oxide;    Au nanoparticles;    Molecularly imprinted electrochemical sensor;    Electrochemical determination;   

DOI  :  10.1016/j.talanta.2016.12.040

来源: Elsevier

【 摘 要 】

An electrochemical sensor for n-mannitol based on molecularly imprinted polymer on electrode modified with reduced graphene oxide decorated with gold nanoparticles was developed in this present work. The sensor was constructed for the first time via the electropolymerization of o-phenylenediamine (o-PD) over a surface containing reduced graphene oxide (RGO) and gold nanoparticles (AuNP) in the presence of n-mannitol molecules. The surface modification with AuNP/RGO-GCE facilitated the charge transfer processes of [Fe(CN)(6)](3-/4-), which was used as an electrochemical probe. It also contributed meaningfully towards the increase in the surface/volume ratio, creating more locations for imprinting, and providing greater sensitivity to the sensor. The 1VIIP/AuNP/RGO-GCE sensor was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM), atomic force microscope (AFM) and X-ray Photoelectron Spectroscopy (XPS). Important parameters that exert control over the performance of the molecularly imprinted sensor (such as number of cycles, pH, monomer and template concentration and extraction and rebinding conditions) were investigated and optimized. The imprinting factor was 4.9, showing greater response to the n-mannitol molecule compared to the interfering molecules. The limit of detection, limit of quantification and amperometric sensitivity were 7.7x10(-13) mol L-1, 2.6x10(-12) mol L-1 and 3.9x10(10) mu A L mol(-1) (n=3) respectively. The MIP/AuNP/RGO-GCE sensor was successfully applied towards the selective determination of n-mannitol in sugarcane vinasse, thus making it, in essence, a valuable tool for the accurate and reliable determination of this molecule.

【 授权许可】

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