Rapid and sensitive identification of uropathogenic Escherichia coli using a surface-enhanced-Raman-scattering-based biochip

TALANTA

卷:219

Article

Andrei, Cristina-Cassiana¹  Moraillon, Anne¹  Lau, Stephanie²  Felidj, Nordin²  Yamakawa, Nao^3,4  Bouckaert, Julie^3,4  Larquet, Eric¹  Boukherroub, Rabah⁵  Ozanam, Francois¹  Szunerits, Sabine⁵  Gouget-Laemmel, Anne Chantal¹ 

[1] Ecole Polytech, Lab Phys Matiere Condensee, CNRS, IP Paris, F-91128 Palaiseau, France

[2] Univ Paris, ITODYS, CNRS, UMR 7086, 15 Rue JA de Baif, F-75013 Paris, France

[3] CNRS, UMR 8576, Unite Glycobiol Struct & Fonct UGSF, 50 Ave Halley, F-59658 Villeneuve Dascq, France

[4] Univ Lille, 50 Ave Halley, F-59658 Villeneuve Dascq, France

[5] Univ Polytech Hauts France, UMR 8520, IEMN, Univ Lille,CNRS,Cent Lille, F-59000 Lille, France

关键词: Biochips;    Surface-enhanced Raman spectroscopy (SERS);    Gold nanorods (Au NRs);    Escherichia coli;    Principle component analysis (PCA);    Hydrogenated amorphous silicon (a-Si:H);   

DOI  :  10.1016/j.talanta.2020.121174

来源: Elsevier

【 摘 要 】

Rapid, selective and sensitive sensing of bacteria remains challenging. We report on a highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS)-based sensing approach for the detection of uropathogenic Escherichia coli (E. coli) bacteria in urine. The assay is based on the specific capture of the bacteria followed by interaction with cetyltrimethylammonium bromide (CTAB)-stabilised gold nanorods (Au NRS) as SERS markers. High sensitivity up to 10 CFU mL(-1) is achieved by optimizing the capture interface based on hydrogenated amorphous silicon a-Si:H thin films. The integration of CH3O-PEG750 onto a-Si:H gives the sensing interface an efficient anti-fouling character, while covalent linkage of antibodies directed against the major type-1 fimbrial pilin FimA of the human pathogen E. coli results in the specific trapping of fimbriated E. coli onto the SERS substrate and their spectral fingerprint identification.

【 授权许可】

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