Quantum dots encoded Au coated polystyrene bead arranged micro-channel for multiplex arrays

TALANTA

卷:146

Article

Cao, Yuan-Cheng^1,2  Wang, Zhan³  Yang, Runyu¹  Zou, Linling^1,2  Zhou, Zhen¹  Mi, Tie⁴  Shi, Hong¹ 

[1] Jianghan Univ, Minist Educ, Key Lab Optoelect Chem Mat & Devices, Wuhan 430056, Peoples R China

[2] Jianghan Univ, Flexible Display Mat & Technol Coinnovat Ctr Hube, Wuhan 430056, Peoples R China

[3] Wuhan Polytech Univ, Dept Chem & Environm Engn, Wuhan 430023, Hubei Province, Peoples R China

[4] Jianghan Univ, Hubei Key Lab Ind Fume & Dust Pollut Control, Wuhan 430056, Peoples R China

关键词: Quantum dots;    Multiplex;    Capillary immunoassays;    Biochips;    Fluorescent analysis;   

DOI  :  10.1016/j.talanta.2015.06.030

来源: Elsevier

【 摘 要 】

This paper describes a promising micro-channel multiplex immunoassay method based on the quantum dots encoded beads which requires micro-volume sample. Briefly, Au nanoparticles coated polystyrene (PS) beads were prepared and Quantum dots (QDs) were employed to encode 4 types of the PS beads by different emission wavelength QDs and various intensities. Different coding types of the beads were immobilized with different antibodies on the surface and BSA was used to block the unsatisfied sites. The antibody linked beads were then arranged in the 150 gm diameter optical capillary where the specific reactions took place before the detections. Results showed that the antibody on the Au coated surface maintains the bioactivity for the immunoreactions. Using this system, the fluorescent intensity was linear with analyte concentration in the range of 1 x 10(-7)-1 x 10(-5) mg/mL (RSD < 5%, 4 repeats) and the lower detection limit reached 5 x 10(-8) mg/mL. It was proved to be a promising approach for the future miniaturization analytical devices. (C) 2015 Elsevier B.V. All rights reserved.

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