Interaction of a laminin derived peptide with phosphatidyl choline/phosphatidyl glycerol

TALANTA

卷:60

Article; Proceedings Paper

Reig, F ; Sospedra, P ; Juvé, A ; Rodriguez, L

关键词: laminin;    phosphatidyl choline;    phosphatidyl glycerol;   

DOI  :  10.1016/S0039-9140(03)00186-3

来源: Elsevier

【 摘 要 】

The physicochemical interactions between an active peptide sequence derived from laminin and phospholipids have been studied. The main aim is to determine the suitability of this peptide fragment to be linked to liposome's with the purpose to develop targeting devices. Results indicate that this peptide is able to insert in lipid monolayers and also to form monomolecular layers at an air/water interface. Besides, miscibility studies carried out through compression isotherms of mixed monolayers [dipalmitoyl phosphatidylcholine (DPPC)/peptide], indicate a strong interaction at 60-80% DPPC molar composition. Studies carried out with lipid bilayers indicate that the interaction is restricted to the external face of the vesicles. Moreover, the presence of this peptide in the incubation media promotes a low level of carboxyfluorescein (CF) leakage and no fusion of vesicles. These results indicate that the association of this sequence to vesicles do not produce damage of the bilayer and can be used as potential targeting vector. (C) 2003 Elsevier Science B.V. All rights reserved.

【 授权许可】

Free