A colorimetric assay system for dopamine using microfluidic paper-based analytical devices

TALANTA

卷:194

Article

Liu, Chunye¹  Gomez, Frank A.²  Miao, Yanqing¹  Cui, Ping¹  Lee, Wilson² 

[1] Xian Med Univ, Sch Pharm, Xian 7100021, Shaanxi, Peoples R China

[2] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA

关键词: Microfluidic paper-based analytical devices;    Colorimetry;    Dopamine;    Oxidation-reduction;   

DOI  :  10.1016/j.talanta.2018.10.039

来源: Elsevier

【 摘 要 】

We report the colorimetric detection of dopamine (DA) on microfluidic paper-based analytical devices (mu PADs) using an oxidation-reduction method. Here, dopamine reacts with ferric chloride forming reduced Fe2+ that subsequently reacts with phenanthroline to form the red tris(1,10-phenanthroline)iron(II) complex. The devices were fabricated by wax printing and changes in color intensity were recorded using a common cell phone. Subsequent analysis using Photoshop software, yielded a limit of detection (LOD) for DA of 0.37 mu mol/L with a linear range of 0.527-4.75 mu mol/L and relative standard deviation of 0.11% (inter-day) and 0.15% (intra-day) for n = 15 paper chips. The effects of detection conditions have been investigated and are discussed. Cow serum samples and human blood serum and plasma samples were detected. The work, herein, demonstrates the potential of this method as a low cost and rapid colorimetric technique to detect DA in real samples.

【 授权许可】

Free