A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

Biosensors and Bioelectronics: X

Jiangshan Wang¹  Andres Dextre¹  Suraj Mohan¹  Jordan Seville¹  Fujr Osman Ibrahim Osman¹  Josiah Levi Davidson²  Ana Pascual-Garrigos²  Murali Kannan Maruthamuthu²  Mohit S. Verma²  Sai Venkata Sravan Putikam²  Darby McChesney² 

[1] Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA;Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA;

关键词: Paper-based diagnostics;    Colorimetric LAMP;    SARS-CoV-2;    Saliva;    Microfluidic paper-based analytical devices;   

DOI  :  

来源: DOAJ

【 摘 要 】

Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.

【 授权许可】

Unknown