Dynamic monitoring and quantitative characterization of intracellular H2O2 content by using SERS based boric acid nanoprobe

TALANTA

卷:214

Article

Cheng, Wendai¹  Tang, Ping¹  He, Xuanmeng^1,2  Xing, Xinyue¹  Xing, Meishuang¹  Lu, Liupeng¹  Ju, Peng¹  Wang, Yi¹  Liu, Shengde¹  Lu, Xiaoxu¹  Zhong, Liyun¹ 

[1] South China Normal Univ, Guangdong Prov Key Lab Nanophoton Funct Mat & Dev, Guangzhou 510006, Peoples R China

[2] South China Normal Univ, Brain Acad, Guangzhou 510631, Peoples R China

关键词: Surface enhanced Raman scattering (SERS);    Boric acid;    Nanoprobe;    Raman spectral imaging;    Cell apoptosis;    Paclitaxel;   

DOI  :  10.1016/j.talanta.2020.120863

来源: Elsevier

【 摘 要 】

Quantitative characterization of intracellular H2O2 content, which is still difficult by the conventional biochemical methods due to the lack of real-time and non-invasive technique of single cell measurement, is a useful solution for cell state assessment. Based on the surface enhanced Raman scattering (SERS), we construct a novel boric acid (BA) nanoprobe to perform quantitative characterization of H2O2 content, in which the p-thiol benzene boric acid (4-MPBA) reporter molecule modified with gold nanorods (AuNRs) is employed for Raman signal enhancement. The achieved result demonstrates obvious advantages of the synthesized AuNRs/4-MPBA/BA nanoprobe in measurement sensitivity of H2O2 content. Importantly, this AuNRs/4-MPBA/BA nanoprobe will provide a powerful tool for dynamic monitoring and quantitative characterization of intracellular H2O2 content during cell apoptosis or other cell growth processes, and then achieve important reference data for studying the corresponding molecular mechanism.

【 授权许可】

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