Novel microfluidic paper-based analytical devices (μPADs) for the determination of nitrate and nitrite in human saliva

TALANTA

卷:219

Article

Ferreira, Francisca T. S. M.¹  Mesquita, Raquel B. R.¹  Rangel, Antonio O. S. S.¹ 

[1] Univ Catolica Portuguesa, CBQF Ctr Biotecnol & Quim Fina, Lab Associado, Escola Super Biotecnol, Rua Diogo Botelho 1327, P-4169005 Porto, Portugal

关键词: Nitrite determination;    Nitrate reduction;    Saliva analysis;    Point-of-care;    Disposable devices;   

DOI  :  10.1016/j.talanta.2020.121183

来源: Elsevier

【 摘 要 】

In this work, two different microfluidic paper-based analytical devices (mu PADs) were developed for the quantification of nitrite and nitrate in human saliva samples, in order to aid in the diagnosis of some diseases and health conditions associated with these ions. The development of these nitrite and nitrate mu PADs involved several studies to optimize their design and construction, including an interference assessment and stability studies. These mu PADs allowed a nitrite determination in a range of 5-250 mu M with limits of detection and quantification of 0.05 mu M and 0.17 mu M, respectively, and a nitrate determination in the range 0.2-1.2 mM with limits of detection and quantification of 0.08 mM and 0.27 mM, respectively. As for the stability, both of the mu PADs were stable when stored in vacuum at 4 degrees C (the nitrite mu PAD for at least 60 days and the nitrate mu PAD for at least of 14 days) and, after the sample placement, the nitrite and nitrate mu PADs could be scanned within the first 4 and 2 h, respectively. The nitrite mu PAD measurements were compared with the ones obtained from the standard colorimetric method and there were no statistically significant differences between these two methods. To evaluate the accuracy of nitrate mu PAD measurements, 4 certified water samples were used and recovery studies using saliva samples were performed.

【 授权许可】

Free