Development of N-ferrocenyl(benzoyl)amino-acid esters stationary phase for high performance liquid chromatography

TALANTA

卷:144

Article

Zhou, Xiaohua¹  Li, Xiaole²  Cao, Aijuan¹  Qiao, Lijun¹  Yu, Ajuan¹  Zhang, Shusheng¹  Wu, Yangjie¹ 

[1] Zhengzhou Univ, Coll Chem & Mol Engn, Key Lab Chem Biol & Organ Chem Henan, Zhengzhou 450052, Peoples R China

[2] Jiyuan Vocat & Tech Coll, Met & Chem Dept, Jiyuan 459000, Peoples R China

关键词: Ferrocene stationary phase;    High performance liquid chromatography;    Retention mechanism;    Organophosphorus pesticide;   

DOI  :  10.1016/j.talanta.2015.07.063

来源: Elsevier

【 摘 要 】

A new stationary phase for high-performance liquid chromatography was prepared by covalently bonding N-ferrocenyl(benzoyl)amino-acid esters (L-1) onto silica gel using 3-aminopropyltriethoxysilane as coupling reagent. The structure of new material was characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. The chromatographic behavior of the phase was illustrated in reversed-phase (RP) and normal-phase (NP) modes using polycyclic aromatic hydrocarbons (PAHs), aromatics positional isomers, amines, 5-nitroimidazoles, organophosphorus pesticides and phenols as probes. Multiple mechanisms including hydrophobic, hydrogen bonding, pi-pi, dipole-dipole, charge-transfer and acid-base equilibrium interactions are involved. Thus, multi-interaction mechanisms and mixed-mode separation of the new phase can very likely guarantee its excellent chromatographic performance for the analysis of complex samples. The L-1 AminoSil column was successfully employed for the analysis of organophosphorus pesticides in vegetable. (C) 2015 Elsevier B.V. All rights reserved.

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