4-Chloro-6-pyrimidinylferrocene modified silica gel: A novel multiple-function stationary phase for mixed-mode chromatography

TALANTA

卷:153

Article

Qiao, Lijun¹  Zhou, Xiaohua¹  Zhang, Yanhao¹  Yu, Ajuan¹  Hu, Kai²  Zhang, Shusheng¹  Wu, Yangjie¹ 

[1] Zhengzhou Univ, Key Lab Chem Biol & Organ Chem Henan, Coll Chem & Mol Engn, Zhengzhou 450052, Peoples R China

[2] Henan Univ Tradit Chinese Med, Zhengzhou 450008, Peoples R China

关键词: Ferrocene stationary phase;    Multi-function;    Mixed-mode;    Linear solvation energy relationship;    Retention mechanism;   

DOI  :  10.1016/j.talanta.2016.02.055

来源: Elsevier

【 摘 要 】

A novel multi-function and mixed-mode stationary phase based on 4-chloro-6-pyrimidinylferrocene modified silica (NFcS) was synthesized and characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. Linear solvation energy relationship method was successfully employed to evaluate the new phase with a set of 27 solutes including aromatic and aliphatic compounds. Multiple mechanisms including hydrophobic, pi-pi, hydrogen-bonding, charge-transfer, acid-base equilibrium and anion-exchange interactions are involved. Based on these interactions, successful separation could be achieved among polycyclic aromatic hydrocarbons, mono-substituted benzenes, aromatic amines, phenols, quinolines, pyridines and nucleosides in reversed-phase (RP) or normal-phase (NP) chromatography. Inorganic anions were also shown to be individually separated in anion-exchange chromatography by using the same column. Moreover, the results here also demonstrated that NFcS based stationary phase could effectively reduce the adverse effect of residual silanol in the separation process. Such stationary phase with characteristics of multi-interaction mechanism and mixed-mode separation is potential for the analysis of complex samples. The NFcS column was successfully employed for the analysis of plant growth regulators in Fruit. (C) 2016 Elsevier B.V. All rights reserved.

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