Pyrroloquinoline quinone-dependent glucose dehydrogenase bioelectrodes based on one-step electrochemical entrapment over single-wall carbon nanotubes

TALANTA

卷:232

Review

Quintero-Jaime, Andres Felipe^1,2  Conzuelo, Felipe⁴  Cazorla-Amoros, Diego^2,3  Morallon, Emilia^1,2 

[1] Univ Alicante, Dept Quim Fis, Ap 99, Alicante 03080, Spain

[2] Univ Alicante, Inst Univ Mat Alicante IUMA, Ap 99, Alicante 03080, Spain

[3] Univ Alicante, Dept Quim Inorgan, Ap 99, Alicante 03080, Spain

[4] Ruhr Univ Bochum, Fac Chem & Biochem, Analyt Chem Ctr Electrochem Sci, Univ St 150, D-44780 Bochum, Germany

关键词: Entrapment;    Carbon nanotubes;    Phosphonic acid;    PQQ-GDH;    Biosensor;    Glucose;   

DOI  :  10.1016/j.talanta.2021.122386

来源: Elsevier

【 摘 要 】

Development of effective direct electron transfer is considered an interesting platform to obtain high performance bioelectrodes. Therefore, designing of scalable and cost-effective immobilization routes that promotes correct direct electrical contacting between the electrode material and the redox enzyme is still required. As we present here, electrochemical entrapment of pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on single-wall carbon nanotube (SWCNT)-modified electrodes was carried out in a single step during electrooxidation of para-aminophenyl phosphonic acid (4-APPA) to obtain active bioelectrodes. The adequate interaction between SWCNTs and the enzyme can be achieved by making use of phosphorus groups introduced during the electrochemical co-deposition of films, improving the electrocatalytic activity towards glucose oxidation. Two different procedures were investigated for electrode fabrication, namely the entrapment of reconstituted holoenzyme (PQQ-GDH) and the entrapment of apoenzyme (apo-GDH) followed by subsequent in situ reconstitution with the redox cofactor PQQ. In both cases, PQQ-GDH preserves its electrocatalytic activity towards glucose oxidation. Moreover, in comparison with a conventional drop-casting method, an important enhancement in sensitivity was obtained for glucose oxidation (981.7 +/- 3.5 nA mM(-1)) using substantially lower amounts of enzyme and cofactor (PQQ). The single step electrochemical entrapment in presence of 4-APPA provides a simple method for the fabrication of enzymatic bioelectrodes.

【 授权许可】

Free