Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging

TALANTA

卷:125

Article

Vincke, Damien¹  Miller, Rebecca²  Stassart, Edith²  Otte, Marcel²  Dardenne, Pierre¹  Collins, Matthew³  Wilkinson, Keith⁴  Stewart, John⁵  Baeten, Vincent¹  Pierna, Juan Antonio Fernandez¹ 

[1] Walloon Agr Res Ctr, Valorisat Agr Prod Dept, B-5030 Gembloux, Belgium

[2] Univ Liege, Serv Prehist, B-4000 Liege, Belgium

[3] Univ York, Dept Archaeol, York YO10 5DD, N Yorkshire, England

[4] Univ Winchester, Dept Archaeol, Winchester SO22 4NR, Hants, England

[5] Bournemouth Univ, Fac Sci & Technol, Poole BH12 5BB, Dorset, England

关键词: Bone;    Collagen;    Near Infrared (NIR) Hyperspectral Imaging;    Principal Component Analysis (PCA);    Partial Least Squares-Discriminant Analysis (PLS-DA);    Sorting;   

DOI  :  10.1016/j.talanta.2014.02.044

来源: Elsevier

【 摘 要 】

The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou Al'Wesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum. (C) 2014 Elsevier B.V. All rights reserved.

【 授权许可】

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